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pcmv6 xl4 tnip2  (OriGene)
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OriGene pcmv6 xl4 tnip2
A Schematic illustrating the protein array screen for MEKK1 PHD substrates using UBE1, UBE2N:UBE2V1 and MEKK1 PHD in a ubiquitination assay. B Ubiquitination assays were performed comprising UBE1, UBE2N:UBE2V1 and MEKK1 PHD or UBE1 and UBE2N:UBE2V1 in the presence of biotin-Ub and profiled using Protoarray Human Protein Microarrays v.5. C A heat map comparing hits between UBE1 + UBE2N:UBE2V1 and UBE1 + UBE2N:UBE2V1 + MEKK1 PHD reactions. D–H Ubiquitination of (D) TAB1, (E) TNIP1, (F) <t>TNIP2,</t> (G) TRAF2, and (H) STAM1 by MEKK1. HEK 293 cells were transfected as indicated, lysates prepared and analysed by IP and IB with the indicated antibodies. Data information: Results are representative of three independent experiments. Source data are available online for this figure.
Pcmv6 Xl4 Tnip2, supplied by OriGene, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Schematic illustrating the protein array screen for MEKK1 PHD substrates using UBE1, UBE2N:UBE2V1 and MEKK1 PHD in a ubiquitination assay. B Ubiquitination assays were performed comprising UBE1, UBE2N:UBE2V1 and MEKK1 PHD or UBE1 and UBE2N:UBE2V1 in the presence of biotin-Ub and profiled using Protoarray Human Protein Microarrays v.5. C A heat map comparing hits between UBE1 + UBE2N:UBE2V1 and UBE1 + UBE2N:UBE2V1 + MEKK1 PHD reactions. D–H Ubiquitination of (D) TAB1, (E) TNIP1, (F) TNIP2, (G) TRAF2, and (H) STAM1 by MEKK1. HEK 293 cells were transfected as indicated, lysates prepared and analysed by IP and IB with the indicated antibodies. Data information: Results are representative of three independent experiments. Source data are available online for this figure.

Journal: The EMBO Journal

Article Title: The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines

doi: 10.15252/embj.201488351

Figure Lengend Snippet: A Schematic illustrating the protein array screen for MEKK1 PHD substrates using UBE1, UBE2N:UBE2V1 and MEKK1 PHD in a ubiquitination assay. B Ubiquitination assays were performed comprising UBE1, UBE2N:UBE2V1 and MEKK1 PHD or UBE1 and UBE2N:UBE2V1 in the presence of biotin-Ub and profiled using Protoarray Human Protein Microarrays v.5. C A heat map comparing hits between UBE1 + UBE2N:UBE2V1 and UBE1 + UBE2N:UBE2V1 + MEKK1 PHD reactions. D–H Ubiquitination of (D) TAB1, (E) TNIP1, (F) TNIP2, (G) TRAF2, and (H) STAM1 by MEKK1. HEK 293 cells were transfected as indicated, lysates prepared and analysed by IP and IB with the indicated antibodies. Data information: Results are representative of three independent experiments. Source data are available online for this figure.

Article Snippet: Anti-CD3 (555273) and anti-CD28 (347690) antibodies were from BD Biosciences. pCMV-Myc mouse MEKK1 and pCMV-Myc mouse MEKK1 C438A, I440A plasmids were purchased from GenScript. pCMV6-XL4 TRAF2, pCMV6-XL4 TNIP2 and pCMV6-Myc TNIP1 were from Origene. pCMV TAB1 and pCMV mTAB1 were purchased from GenScript.

Techniques: Protein Array, Ubiquitin Assay, Transfection